| Experiment ID | EXP00272 |
| Reference | Title: MicroRNA expression profile in head and neck cancer: HOX-cluster embeddedmicroRNA-196a and microRNA-10b dysregulation implicated in cell proliferation. Author: Severino P, Brüggemann H, Andreghetto FM, Camps C, Klingbeil Mde F, de PereiraWO, Soares RM, Moyses R, Wünsch-Filho V, Mathor MB, Nunes FD, Ragoussis J, TajaraEH. Journal: BMC Cancer. 2013 Nov 9;13:533. doi: 10.1186/1471-2407-13-533. Abstract: BACKGROUND: Current evidence implicates aberrant microRNA expression patterns in human malignancies; measurement of microRNA expression may have diagnostic andprognostic applications. Roles for microRNAs in head and neck squamous cellcarcinomas (HNSCC) are largely unknown. HNSCC, a smoking-related cancer, is oneof the most common malignancies worldwide but reliable diagnostic and prognostic markers have not been discovered so far. Some studies have evaluated thepotential use of microRNA as biomarkers with clinical application in HNSCC.METHODS: MicroRNA expression profile of oral squamous cell carcinoma samples was determined by means of DNA microarrays. We also performed gain-of-function assaysfor two differentially expressed microRNA using two squamous cell carcinoma cell lines and normal oral keratinocytes. The effect of the over-expression of thesemolecules was evaluated by means of global gene expression profiling and cellproliferation assessment.RESULTS: Altered microRNA expression was detected for a total of 72 microRNAs.Among these we found well studied molecules, such as the miR-17-92 cluster,comprising potent oncogenic microRNA, and miR-34, recently found to interact withp53. HOX-cluster embedded miR-196a/b and miR-10b were up- and down-regulated,respectively, in tumor samples. Since validated HOX gene targets for thesemicroRNAs are not consistently deregulated in HNSCC, we performedgain-of-function experiments, in an attempt to outline their possible role. Ourresults suggest that both molecules interfere in cell proliferation throughdistinct processes, possibly targeting a small set of genes involved in cellcycle progression.CONCLUSIONS: Functional data on miRNAs in HNSCC is still scarce. Our datacorroborate current literature and brings new insights into the role of microRNAsin HNSCC. We also show that miR-196a and miR-10b, not previously associated with HNSCC, may play an oncogenic role in this disease through the deregulation ofcell proliferation. The study of microRNA alterations in HNSCC is an essentialstep to the mechanistic understanding of tumor formation and could lead to thediscovery of clinically relevant biomarkers. PMID: 24209638 |
| Expressiion Profile | Description: MicroRNA expression profile in Head and Neck Cancer: HOX-cluster embedded microRNA-196 and microRNA-10b dysregulation is implicated in cell proliferation Organism: Homo sapiens GEO ID: GSE31277 Platform: GPL9770 Number of samples: 42 |
| Design and Sample | Cancer Type: head and neck cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: head and neck cancer Control Sample: normal tissue Num of Case: 15 Num of Control: 15 Quantification Software: Limma Num of miRNAs: 470 |
| Identification | Num of Up: 45 Num of Down: 84 |