| Experiment ID | EXP00276 |
| Reference | Title: Regulatory roles of miRNA in the human neural stem cell transformation to glioma stem cells. Author: Liu S, Yin F, Zhang J, Wicha MS, Chang AE, Fan W, Chen L, Fan M, Li Q. Journal: J Cell Biochem. 2014 Aug;115(8):1368-80. doi: 10.1002/jcb.24786. Abstract: To investigate the expressional alternation of microRNAs (miRNA) during themalignant transformation and development of human glioma, we measured miRNAexpression profile as well as mRNA expression profile in normal human neural stemcells (hNSCs) and human glioma stem cells (hGSCs). We found 116 miRNAup-regulated and 62 miRNA down-regulated in GSCs. On the other hand, weidentified 1,372 mRNA down-regulated, and 1,501 mRNA up-regulated in GSCscompared to those in NSCs. We then analyzed the pathways and the predicted targetgenes of the miRNAs which differ significantly in expression between GSCs andNSCs using the statistical enrichment methods. These target mRNAs are involved inmany cancer-related signaling pathways, such as cell cycle, axon guidance, gliomadevelopment, adhesion junction, MAPK and Wnt signaling. Furthermore, we obtained the differently expressed miRNA-target relationships according to the θ valuewhich is used to calculate the regulation extent of miRNA-target and using thedatabases of miRanda, Targetscans and Pictar. Among the top 10 miRNA-targetrelationships, hsa-miR-198 and its potential targeted gene DCX and NNAT wereselected for validation, and NNAT was found to be the direct target of miR-198.Finally, the functional roles of miR-155-5p and miR-124-3p whose expressionsaltered significantly between GSCs and NSCs were addressed. Our results providenew clues for the potential mechanisms involved in the origin and development of glioma. Clinically, the altered miRNAs may serve as potential targets anddiagnostic tools for novel therapeutic strategies of glioblastoma. PMID: 24519663 |
| Expressiion Profile | Description: miRNA profiles of brain glioma stem cells and normal neural stem cells Organism: Homo sapiens GEO ID: GSE41032 Platform: GPL15516 Number of samples: 6 |
| Design and Sample | Cancer Type: brain cancer Cancer SubType: glioma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: glioma Control Sample: normal brain Num of Case: 3 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 1658 |
| Identification | Num of Up: 71 Num of Down: 58 |