| Experiment ID | EXP00281 |
| Reference | Title: Downregulated miR329 and miR410 promote the proliferation and invasion of oralsquamous cell carcinoma by targeting Wnt-7b. Author: Shiah SG, Hsiao JR, Chang WM, Chen YW, Jin YT, Wong TY, HuangJS, Tsai ST, Hsu YM, Chou ST, Yen YC, Jiang SS, Shieh YS,Chang IS, Hsiao M, Chang JY. Journal: Cancer Res. 2014 Dec 15;74(24):7560-72. doi: 10.1158/0008-5472.CAN-14-0978. Epub 2014 Oct 28. Abstract: microRNA (miRNA) dysregulation contributes widely to human cancer but has notbeen fully assessed in oral cancers. In this study, we conducted a globalmicroarray analysis of miRNA expression in 40 pairs of betel quid-associated oralsquamous cell carcinoma (OSCC) specimens and their matched nontumorous epithelialcounterparts. Eighty-four miRNAs were differentially expressed in the OSCCspecimens compared with the matched tissue. Among these downregulated miRNAs, 19 miRNAs were found and mapped to the chromosome 14q32.2 miRNA cluster region,which resides within a parentally imprinted region designated as Dlk-Dio3 andknown to be important in development and growth. Bioinformatic analysis predictedtwo miRNAs from the cluster region, miR329 and miR410, which could potentiallytarget Wnt-7b, an activator of the Wnt-β-catenin pathway, thereby attenuating theWnt-β-catenin signaling pathway in OSCC. Stable ectopic expression of Wnt-7b inOSCC cells overexpressing miR329 or miR410 restored proliferation and invasioncapabilities abolished by these miRNA. Combining a demethylation agent and ahistone deacetylase inhibitor was sufficient to reexpress miR329, miR410, andMeg3, consistent with epigenetic regulation of these miRNA in human OSCC.Specifically, arecoline, a major betel nut alkaloid, reduced miR329, miR410, and Meg3 gene expression. Overall, our results provide novel molecular insights into how betel quid contributes to oral carcinogenesis through epigenetic silencing oftumor-suppressor miRNA that targets Wnt-β-catenin signaling. PMID: 25351956 |
| Expressiion Profile | Description: Identification of oral carcinoma miRNA involved in Wnt/b-catenin signaling Organism: Homo sapiens GEO ID: GSE45238 Platform: GPL8179 Number of samples: 80 |
| Design and Sample | Cancer Type: oral squamous cell carcinoma Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: oral squamous cell carcinoma Control Sample: normal tissue Num of Case: 40 Num of Control: 40 Quantification Software: Limma Num of miRNAs: 846 |
| Identification | Num of Up: 181 Num of Down: 178 |