| Experiment ID | EXP00283 |
| Reference | Title: MicroRNA profile in very young women with breast cancer. Author: Peña-Chilet M, Martínez MT, Pérez-Fidalgo JA, Peiró-Chova L, Oltra SS, Tormo E,Alonso-Yuste E, Martinez-Delgado B, Eroles P, Climent J, Burgués O, Ferrer-LozanoJ, Bosch A, Lluch A, Ribas G. Journal: BMC Cancer. 2014 Jul 21;14:529. doi: 10.1186/1471-2407-14-529. Abstract: BACKGROUND: Breast cancer is rarely diagnosed in very young women (35 years oldor younger), and it often presents with distinct clinical-pathological featuresrelated to a more aggressive phenotype and worse prognosis when diagnosed at thisearly age. A pending question is whether breast cancer in very young women arisesfrom the deregulation of different underlying mechanisms, something that willmake this disease an entity differentiated from breast cancer diagnosed in older patients.METHODS: We performed a comprehensive study of miRNA expression using miRNAAffymetrix2.0 array on paraffin-embedded tumour tissue of 42 breast cancerpatients 35 years old or younger, 17 patients between 45 and 65 years old and 29 older than 65 years. Data were statistically analyzed by t-test and ahierarchical clustering via average linkage method was conducted. Results werevalidated by qRT-PCR. Putative targeted pathways were obtained using DIANAmiRPath online software.RESULTS: The results show a differential and unique miRNA expression profile of121 miRNAs (p-value <0.05), 96 of those with a FDR-value <0.05. Hierarchicalclustering grouped the samples according to their age, but not by subtype nor by tumour characteristics. We were able to validate by qRT-PCR differences in theexpression of 6 miRNAs: miR-1228*, miR-3196, miR-1275, miR-92b, miR-139 andmiR-1207. Moreover, all of the miRNAs maintained the expression trend. Thevalidated miRNAs pointed out pathways related to cell motility, invasion andproliferation.CONCLUSIONS: The study suggests that breast cancer in very young women appears asa distinct molecular signature. To our knowledge, this is the first time that avalidated microRNA profile, distinctive to breast cancer in very young women, hasbeen presented. The miRNA signature may be relevant to open an important field ofresearch in order to elucidate the underlying mechanism in this particulardisease, which in a more clinical setting, could potentially help to identifytherapeutic targets in this particular set of patients. PMID: 25047087 |
| Expressiion Profile | Description: Expression of miRNAs in breast tumor from very youn women (<36) and women above 65 years old Organism: Homo sapiens GEO ID: GSE48088 Platform: GPL14613 Number of samples: 36 |
| Design and Sample | Cancer Type: breast cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: breast cancer Control Sample: normal breast Num of Case: 5 Num of Control: 41 Quantification Software: Limma Num of miRNAs: 1105 |
| Identification | Num of Up: 5 Num of Down: 41 |