| Experiment ID | EXP00290 |
| Reference | Title: Distinct miRNA signatures associate with subtypes of cholangiocarcinoma frominfection with the tumourigenic liver fluke Opisthorchis viverrini. Author: Plieskatt JL, Rinaldi G, Feng Y, Peng J, Yonglitthipagon P, EasleyS, Laha T, Pairojkul C, Bhudhisawasdi V, Sripa B, Brindley PJ, Mulvenna JP, Bethony JM. Journal: J Hepatol. 2014 Oct;61(4):850-8. doi: 10.1016/j.jhep.2014.05.035. Epub 2014 Jun3. Abstract: BACKGROUND & AIMS: Intrahepatic cholangiocarcinoma (ICC) is a significant public health problem in East Asia, where it is strongly associated with chronicinfection by the food-borne parasite Opisthorchis viverrini (OV). We report thefirst comprehensive miRNA expression profiling by microarray of the most commonhistologic grades and subtypes of ICC: well differentiated, moderatelydifferentiated, and papillary ICC.METHODS: MicroRNA expression profiles from FFPE were compared among thefollowing: ICC tumour tissue (n = 16), non-tumour tissue distally macrodissected from the same ICC tumour block (n = 15), and normal tissue (n = 13) fromindividuals undergoing gastric bypass surgery. A panel of deregulated miRNAs was validated by qPCR.RESULTS: Each histologic grade and subtype of ICC displayed a distinct miRNAprofile, with no cohort of miRNAs emerging as commonly deregulated. Moderatelydifferentiated ICC showed the greatest miRNA deregulation in quantity andmagnitude, followed by the papillary subtype, and then well differentiated ICC.Moreover, when ICC tumour tissues were compared to adjacent non-tumour tissue,similar miRNA dysregulation profiles were observed.CONCLUSIONS: We show that common histologic grades and subtypes of ICC havedistinct miRNA profiles. As histological grade and subtypes are associated withICC aggressiveness, these profiles could be used to enhance the early detectionand improve the personalised treatment for ICC. These findings also suggest theinvolvement of specific miRNAs during ICC tumour progression and differentiation.We plan to use these insights to (a) detect these profiles in circulation and (b)conduct functional analyses to decipher the roles of miRNAs in ICC tumourdifferentiation. PMID: 25017828 |
| Expressiion Profile | Description: The miRNAome of O. viverrini induced cholangiocarcinoma by microarray Organism: Homo sapiens GEO ID: GSE53992 Platform: GPL18159 Number of samples: 46 |
| Design and Sample | Cancer Type: biliary tract cancer Cancer SubType: cholangiocarcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: cholangiocarcinoma Control Sample: normal intrahepatic bile duct Num of Case: 33 Num of Control: 13 Quantification Software: Limma Num of miRNAs: 1205 |
| Identification | Num of Up: 129 Num of Down: 174 |