| Experiment ID | EXP00296 |
| Reference | Title: Molecular signatures of mRNAs and miRNAs as prognostic biomarkers inpancreatobiliary and intestinal types of periampullary adenocarcinomas. Author: Sandhu V, Bowitz Lothe IM, Labori KJ, Lingjærde OC, Buanes T,Dalsgaard AM, Skrede ML, Hamfjord J, Haaland T, Eide TJ,Børresen-Dale AL, Ikdahl T, Kure EH. Journal: Mol Oncol. 2015 Apr;9(4):758-71. doi: 10.1016/j.molonc.2014.12.002. Epub 2014 Dec19. Abstract: Periampullary adenocarcinomas include four anatomical sites of origin (thepancreatic duct, bile duct, ampulla and duodenum) and most of them fall into two histological subgroups (pancreatobiliary and intestinal). Determining the exactorigin of the tumor is sometimes difficult, due to overlapping histopathological characteristics. The prognosis depends on the histological subtype, as well as onthe anatomical site of origin, the former being the more important. The molecularbasis for these differences in prognosis is poorly understood. Whole-genomeanalyses were used to investigate the association between molecular tumorprofiles, pathogenesis and prognosis. A total of 85 periampullary adenocarcinomaswere characterized by mRNA and miRNA expressions profiling. Molecular profiles ofthe tumors from the different anatomical sites of origin as well as of thedifferent histological subtypes were compared. Differentially expressed mRNAs andmiRNAs between the two histopathological subtypes were linked to specificmolecular pathways. Six miRNA families were downregulated and four wereupregulated in the pancreatobiliary type as compared to the intestinal type (P < 0.05). miRNAs and mRNAs associated with improved overall and recurrence freesurvival for the two histopathological subtypes were identified. For thepancreatobiliary type the genes ATM, PTEN, RB1 and the miRNAs miR-592 andmiR-497, and for the intestinal type the genes PDPK1, PIK3R2, G6PC and the miRNAsmiR-127-3p, miR-377* were linked to enriched pathways and identified asprognostic markers. The molecular signatures identified may in the future guidethe clinicians in the therapeutic decision making to an individualized treatment,if confirmed in other larger datasets. PMID: 25579086 |
| Expressiion Profile | Description: mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA] Organism: Homo sapiens GEO ID: GSE60978 Platform: GPL15159 Number of samples: 89 |
| Design and Sample | Cancer Type: pancreatic cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: pancreatic ductal adenocarcinoma Control Sample: normal tissue Num of Case: 51 Num of Control: 6 Quantification Software: Limma Num of miRNAs: 1205 |
| Identification | Num of Up: 89 Num of Down: 166 |