| Experiment ID | EXP00329 |
| Reference | Title: Potentiality of a triple microRNA classifier: miR-193a-3p, miR-23a and miR-338-5pfor early detection of colorectal cancer. Author: Yong FL, Law CW, Wang CW. Journal: BMC Cancer. 2013 Jun 8;13:280. doi: 10.1186/1471-2407-13-280. Abstract: BACKGROUND: MicroRNAs (miRNAs) are short, non-coding RNA molecules that act asregulators of gene expression. Circulating blood miRNAs offer great potential as cancer biomarkers. The objective of this study was to correlate the differential expression of miRNAs in tissue and blood in the identification of biomarkers for early detection of colorectal cancer (CRC).METHODS: The study was divided into two phases: (I) Marker discovery by miRNAmicroarray using paired cancer tissues (n = 30) and blood samples (CRC, n = 42;control, n = 18). (II) Marker validation by stem-loop reverse transcription real time PCR using an independent set of paired cancer tissues (n = 30) and bloodsamples (CRC, n = 70; control, n = 32). Correlation analysis was determined byPearson's test. Logistic regression and receiver operating characteristics curve analyses were applied to obtain diagnostic utility of the miRNAs.RESULTS: Seven miRNAs (miR-150, miR-193a-3p, miR-23a, miR-23b, miR-338-5p,miR-342-3p and miR-483-3p) have been found to be differentially expressed in bothtissue and blood samples. Significant positive correlations were observed in the tissue and blood levels of miR-193a-3p, miR-23a and miR-338-5p. Moreover,increased expressions of these miRNAs were detected in the more advanced stages. MiR-193a-3p, miR-23a and miR-338-5p were demonstrated as a classifier for CRCdetection, yielding a receiver operating characteristic curve area of 0.887(80.0% sensitivity, 84.4% specificity and 83.3% accuracy).CONCLUSION: Dysregulations in circulating blood miRNAs are reflective of those incolorectal tissues. The triple miRNA classifier of miR-193a-3p, miR-23a andmiR-338-5p appears to be a potential blood biomarker for early detection of CRC. PMID: 23758639 |
| Expressiion Profile | Description: Tissue and blood microRNA array profiles of colorectal cancer patients Organism: Homo sapiens GEO ID: GSE39845 Platform: GPL14613 Number of samples: 26 |
| Design and Sample | Cancer Type: colon cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: blood Case Sample: blood from colon cancer Control Sample: normal blood Num of Case: 14 Num of Control: 6 Quantification Software: limma Num of miRNAs: 1105 |
| Identification | Num of Up: 3 Num of Down: 0 |