| Experiment ID | EXP00330 |
| Reference | Title: Identification and Insilico Analysis of Retinoblastoma Serum microRNA Profile andGene Targets Towards Prediction of Novel Serum Biomarkers. Author: Beta M, Venkatesan N, Vasudevan M, Vetrivel U, Khetan V, Krishnakumar S. Journal: Bioinform Biol Insights. 2013;7:21-34. doi: 10.4137/BBI.S10501. Epub 2013 Jan 9. Abstract: Retinoblastoma (RB) is a malignant tumor of the retina seen in children, andpotential non invasive biomarkers are in need for rapid diagnosis and forprognosticating the therapy. This study was undertaken to identify thedifferentially expressed miRNAs in the serum of children with RB in comparisonwith the normal age matched serum, to analyze its concurrence with the existingRB tumor miRNA profile, to identify its novel gene targets specific to RB, and tostudy the expression of a few of the identified oncogenic miRNAs in the advanced stage primary RB patient's serum sample. MiRNA profiling was performed on 14pooled serum from children with advanced RB and 14 normal age matched serumsamples, wherein 21 miRNAs were found to be upregulated (fold change ≤ -2.0, P ≤ 0.05) and 24 to be downregulated (fold change ≥ +2.0, P ≤ 0.05). Furthermore,intersection of 59 significantly deregulated miRNAs identified from RB tumorprofiles with that of miRNAs detected in serum profile revealed that 33 miRNAshad followed a similar deregulation pattern in RB serum. Later we validated a fewof the miRNAs (miRNA 17-92) identified by microarray in the RB patient serumsamples (n = 20) by using qRT-PCR. Expression of the oncogenic miRNAs, miR-17,miR-18a, and miR-20a by qRT-PCR was significant in the serum samples exploringthe potential of serum miRNAs identification as noninvasive diagnosis. Moreover, from miRNA gene target prediction, key regulatory genes of cell proliferation,apoptosis, and positive and negative regulatory networks involved in RBprogression were identified in the gene expression profile of RB tumors.Therefore, these identified miRNAs and their corresponding target genes couldgive insights on potential biomarkers and key events involved in the RB pathway. PMID: 23400111 |
| Expressiion Profile | Description: Differential microRNA expression profiles in Retinoblastoma serum Organism: Homo sapiens GEO ID: GSE41321 Platform: GPL148767 Number of samples: 6 |
| Design and Sample | Cancer Type: retinoblastoma Cancer SubType: N/D Cell Line: N/D Experimental Design: blood Case Sample: blood from retinoblastoma Control Sample: normal blood Num of Case: 3 Num of Control: 3 Quantification Software: limma Num of miRNAs: 851 |
| Identification | Num of Up: 94 Num of Down: 70 |